EFEK MINUMAN BERENERGI TERHADAP HISTOPATOLOGI HEPAR TIKUS WISTAR (Rattus norvegicus)

Mohammad Farhan Aditya, Kasiyati Kasiyati, Sri Isdadiyanto, Silvana Tana, Sunarno Sunarno

Abstract


Minuman berenergi merupakan minuman ringan yang dapat meningkatkan energi, dan mengurangi kelelahan. Kebiasaan mengonsumsi minuman berenergi secara berlebihan dapat meningkatkan resiko kerusakan organ, terutama hepar. Tujuan dilakukannya penelitian ini adalah untuk mengkaji efek minuman berenergi dalam level dosis berbeda terhadap histopatologi hepar tikus wistar. Kandungan kafein, niasin, dan aspartam dalam minuman berenergi merupakan pemicu terjadinya kerusakan pada hepar. Penelitian ini menggunakan desain Rancangan Acak Lengkap dengan 20 ekor tikus putih jantan dengan bobot ±200 gram, yang dibagi dalam 5 perlakuan dengan 4 kali ulangan. Perlakuan terdiri atas P0, P1, P2, P3 dan P4, yang secara berurutan adalah kontrol, tikus putih diberikan minuman berenergi 76 mg/ 200 g BB/ hari, 152 mg/ 200 g BB/ hari, 228 mg/ 200 g BB/ hari, dan 304 mg/ 200 g BB/ hari. Variabel penelitian yang diukur, meliputi bobot hepar, bobot badan, konsumsi pakan, konsumsi minum, hepatosomatik index (HI index), dan diameter hepatosit. Data yang diperoleh dianalisis dengan uji Analysis of Variance (ANOVA), dilanjutkan dengan uji Duncan pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa minuman berenergi memberi efek nyata (P<0,05) terhadap diameter sel hepatosit, bobot badan, dan hepatosomatic indeks, namun tidak berbeda nyata terhadap bobot hepar, konsumsi pakan, dan konsumsi minum. Kesimpulan dari penelitian ini adalah, minuman berenergi dapat merusak histologi hepar tikus wistar. Histologi hepar tikus mulai mengalami kerusakan saat diberikan perlakuan minuman berenergi dengan dosis 76 mg/200 g BB/hari yang ditandai oleh adanya kongesti dan inklusi Mallory Body pada jaringan hepar

Keywords


Minuman Berenergi, Tikus Putih, Hepar & Histopatologi

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DOI: https://doi.org/10.33758/mbi.v15i7.952

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